Abstract
SYNOPSISHistochemical methods were used to determine the basis of aldehyde fuchsin staining in the following tissue constituents: the preoptic and neurohypophysial neurosecretory material of the Bullhead (Cottus gobio) and the pancreatic zymogen granules, gastric‐chief‐cell granules, Paneth‐cell granules, elastic tissue, and acidic mucins of intestinal goblet cells in the mouse.Following oxidation, the staining of neurosecretory material, pancreatic, gastric, and Paneth granules, and elastic tissue is probably due to ionic links with the acidic products of cystine oxidation.Staining occurred without oxidation in pancreatic, gastric, and Paneth‐cell granules and elastin; this reaction is probably non‐ionic, but the chemical groups responsible are still undetermined.Acidic mucins probably stain by ionic linkage to sulphate groups. This confirms previous results.The aldehydes which are artificially produced in the Feulgen and ninhydrin‐Schiff techniques are stained with aldehyde fuchsin but only weakly.
Reference49 articles.
1. Fuchsin‐aldehyde staining of sulfated mucopolysaccharides and related substances;Abul‐Haj S. K.;J. natn. Cancer Inst.,1952
2. THE HYPOTHALAMIC ELABORATION OF POSTERIOR PITUITARY PRINCIPLES IN MAN, THE RAT AND DOG. HISTOCHEMICAL EVIDENCE DERIVED FROM A PERFORMIC ACID-ALCIAN BLUE REACTION FOR CYSTINE
3. The histochemical recognition of lipine;Baker J. R.;Quart. J. micr. Sci.,1946
4. The histochemical recognition of certain guanidine derivatives;Baker J. R.;Quart. J. micr. Sci.,1947
5. Further remarks on the Golgi element;Baker J. R.;Quart. J. micr. Sci.,1949