Abstract
SYNOPSISThe uptake of RNase by two types of amoeba and a selection of vertebrate cells has been investigated using the fluorescein‐labelled enzyme. This method is considered satisfactory for the study since the fluorescein label migrated together with the enzyme during electrophoresis, and the labelled RNase retained one half of its enzymatic activity. Although RNase was ingested as droplets by both Amoeba proteus and Acanthamoeba sp., these two amoebae differed in their extent of surface adsorption of the enzyme and in the effect of RNase on the activity of their contractile vacuoles. Cytoplasmic droplets were also ingested in vitro by a small proportion of ascites cells, but there was no detectable uptake by rabbit kidney, mouse fibroblast, chick embryo or HeLa cultures.
Reference29 articles.
1. Action of Ribonuclease and Ribonucleic Acid on Living Amœbæ
2. Further observations on the action of ribonuclease on living amoebae
3. L'action de la ribonucléase sur la division des oeufs d'amphibiens. 2. Étude cytologique et cytochimique des effets de la ribonucléase chez le pleurodèle;Brachet J.;Exp. Cell Res,1955