Elevated binding activity of CD8+ cells with phytohaemagglutinin by asbestos fibre in vitro

Author:

KINUGAWA K1,HYODOH F1,ANDOH A1,UEKI A1,TANAKA H2,MOCHIZUKI Y1

Affiliation:

1. Department of Hygiene, Kawasaki Medical School, Matsushima, Kurashiki, Japan

2. Department of Urology, Kawasaki Medical School, Matsushima, Kurashiki, Japan

Abstract

SUMMARY Asbestos fibres are known to depress the mitogenic stimuli of phytohaemagglutinin (PHA) to lymphocytes. We examined effects of asbestos (chrysotile) fibre on the proliferation of PHA-stimulated lymphocytes and the PHA binding activity of lymphocytes in vitro. The incorporation of 3H-thymidine and the expression of interleukin-2 receptor were depressed when the cells were exposed to 50 μg/ml of chrysotile fibre. The PHA binding activity of lymphocytes was significantly enhanced after chrysotile fibre exposure, as compared with non-exposed group. These results indicate that the depression of PHA stimuli with chrysotile fibre was not due to blocking of PHA binding to lymphocytes. The enhancement of PHA binding activity by chrysotile fibre was observed on CD8+ but not on CD4+ cells. It is possible, therefore, that treatment of peripheral blood mononuclear cells with chrysotile fibre induces increased reactivity of CDS+ cells with PHA, and that intensely activated CD8+ cells suppress proliferation of lymphocytes.

Publisher

Oxford University Press (OUP)

Subject

Immunology,Immunology and Allergy

Reference35 articles.

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