Effect of chlorpromazine on kinetics of injected monoclonal antibody in MoAb-induced glomerular injury

Abstract

SUMMARY The effect of chlorpromazine, one of several calmodulin antagonists that inhibit cytoskeletal movement, on the local kinetics of injected proteinuria-inducing MoAb 5–1–6 was examined to test the hypothesis that proteinuria is inhibited if the antigen recognized by MoAb 5–1–6 or injected MoAb remains on the surface of epithelial foot processes. MoAb 5–1–6 was injected into both chlorpromazine-treated (5 mg/100 g body weight) and untreated rats. As a positive control for the chlorpromazine treatment, anti-Fx 1A serum was also injected into other chlorpromazine-treated and untreated rats. Chlorpromazine inhibited neither the change in localization of injected MoAb 5–1–6 nor proteinuria, although it showed an inhibitory effect on redistribution of immune complex and the fixation of complement in passive Heymann glomerulonephritis induced by injection of anti-Fx 1A serum. We conclude that the kinetics of bound MoAb 5–1–6 are regulated by a system different from that operating in passive Heymann glomerulonephritis.