HBsAg in urine: a new approach for the detection of urinary antigens-Reference-Cited by-同舟云学术

HBsAg in urine: a new approach for the detection of urinary antigens

Published:1992-02 Issue:2 Volume:87 Page:298-303
ISSN:1365-2249
Container-title:Clinical and Experimental Immunology
language:en
Short-container-title:

Author:

BADUR S¹,GRANGEOT-KEROS L¹,PILLOT J¹²

Affiliation:

1. Service de Microbiologie et d'Immunologie, and INSERM U 131 Hôpital Antoine Béclère, Clamart

2. Unité d'Immunologie Microbienne, Institut Pasteur, Paris, France

Abstract

SUMMARY In order to define the optimal conditions for detection of microbial antigens in urine, urinary HBsAg excreted during hepatitis B was chosen as a model. Using commercial kits, which mainly involve anti-discontinuous epitopes, we found urinary HBsAg in only 50% of patients with HBsAg in their sera. In contrast, with an inhibition method involving a monoclonal antibody recognizing a continuous epitope, urinary HBsAg was found in 100% of these patients. Structural analysis of HBsAg showed that urinary HBsAg is denatured; it can escape detection by commercial kits well fitted for detection of native serum HBsAg. General implications for the revelation of urinary microbial antigens are discussed.

Publisher

Oxford University Press (OUP)

Subject

Immunology,Immunology and Allergy

Link

https://academic.oup.com/cei/article-pdf/87/2/298/42213931/j.1365-2249.1992.tb02991.x.pdf

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