Affiliation:
1. Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark
2. DCCC Brain Tumor Center Copenhagen University Hospital Copenhagen Denmark
3. Department of Pathology, Center of Diagnostic Investigation Copenhagen University Hospital Copenhagen Denmark
4. Section for Bioinformatics, Health Technology Technical University of Denmark (DTU) Denmark
Abstract
AbstractOuter radial glial cells (oRGs) give rise to neurons and glial cells and contribute to cell migration and expansion in developing neocortex. HOPX has been described as a marker of oRGs and possible actor in glioblastomas. Recent years' evidence points to spatiotemporal differences in brain development which may have implications for the classification of cell types in the central nervous system and understanding of a range of neurological diseases. Using the Human Embryonic/Fetal Biobank, Institute of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark, HOPX and BLBP immunoexpression was investigated in developing frontal, parietal, temporal and occipital human neocortex, other cortical areas and brain stem regions to interrogate oRG and HOPX regional heterogeneity. Furthermore, usage of high‐plex spatial profiling (Nanostring GeoMx® DSP) was tested on the same material. HOPX marked oRGs in several human developing brain regions as well as cells in known gliogenic areas but did not completely overlap with BLBP or GFAP. Interestingly, limbic structures (e.g. olfactory bulb, indusium griseum, entorhinal cortex, fimbria) showed more intense HOPX immunoreactivity than adjacent neocortex and in cerebellum and brain stem, HOPX and BLBP seemed to stain different cell populations in cerebellar cortex and corpus pontobulbare. DSP screening of corresponding regions indicated differences in cell type composition, vessel density and presence of apolipoproteins within and across regions and thereby confirming the importance of acknowledging time and place in developmental neuroscience.
Funder
Det Sundhedsvidenskabelige Fakultet, Københavns Universitet
Læge Sophus Carl Emil Friis og hustru Olga Doris Friis' Legat
Vera og Carl Johan Michaelsens Legat
Subject
Cell Biology,Developmental Biology,Molecular Biology,Ecology, Evolution, Behavior and Systematics,Histology,Anatomy
Cited by
3 articles.
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