Two distinct SNARE complexes mediate vesicle fusion with the plasma membrane to ensure effective development and pathogenesis of Fusarium oxysporum f. sp. cubense

Author:

Fang Zhenyu12,Zhao Qiwen12,Yang Shiyu12,Cai Yan12,Fang Wenqin12,Abubakar Yakubu Saddeeq123,Lin Ying14,Yun Yingzi12ORCID,Zheng Wenhui12ORCID

Affiliation:

1. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection Fujian Agriculture and Forestry University Fuzhou Fujian China

2. Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, College of Plant Protection Fujian Agriculture and Forestry University Fuzhou Fujian China

3. Department of Biochemistry, Faculty of Life Sciences Ahmadu Bello University Zaria Nigeria

4. School of Biological and Environmental Engineering Jingdezhen University Jingdezhen Jiangxi China

Abstract

AbstractSNAREs (soluble N‐ethylmaleimide‐sensitive factor attachment protein receptors) facilitate docking and fusion of vesicles with their target membranes, playing a crucial role in vesicle trafficking and exocytosis. However, the spatial assembly and roles of plasma membrane (PM)‐associated SNAREs in phytopathogen development and pathogenicity are not clearly understood. In this study, we analysed the roles and molecular mechanisms of PM‐associated SNARE complexes in the banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4). Our findings demonstrate that FocSso1 is important for the fungal growth, conidiation, host penetration and colonization. Mechanistically, FocSso1 regulates protein secretion by mediating vesicle docking and fusion with the PM and hyphal apex. Interestingly, a FocSso1–FocSec9–FocSnc1 complex was observed to assemble not only at the fungal PM but also on the growing hyphal apex, facilitating exocytosis. FocSso2, a paralogue of FocSso1, was also found to form a ternary SNARE complex with FocSec9 and FocSnc1, but it mainly localizes to the PM in old hyphae. The functional analysis of this protein demonstrated that it is dispensable for the fungal growth but necessary for host penetration and colonization. The other subunits, FocSec9 and FocSnc1, are involved in the fungal development and facilitate host penetration. Furthermore, FocSso1 and FocSnc1 are functionally interdependent, as loss of FocSso1 leads to mis‐sorting and degradation of FocSnc1 in the vacuole and vice versa. Overall, this study provides insight into the formation of two spatially and functionally distinct PM SNARE complexes and their involvement in vesicle exocytosis to regulate development and pathogenicity of FocTR4.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Fujian Province

Publisher

Wiley

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