The correlation between soluble human leukocyte antigen (sHLA‐G) levels and +3010 polymorphism

Abstract

AbstractHuman leukocyte antigen‐G (HLA‐G) is classified as non‐classical HLA, located in the short arm of chromosome 6 and composed of seven introns and eight exons. The HLA‐G gene has a lower frequency polymorphism in the coding area and higher variability at the regulatory 5′‐ and 3′‐untranslated regions linked to HLA‐G microRNA regulation. HLA‐G molecule is known to have an immunomodulatory and tolerogenic features role. In 199 Saudi individuals, we examined the association between plasma soluble HLA‐G (sHLA‐G) levels and eight polymorphic different sites, including 14 bp ins/del/+3003T‐C/+3010C‐G/+3027C‐A/+3035C‐T/+3142C‐G/+3187A‐G/+3196C‐G single nucleotide polymorphisms (SNPs) in exon 8 in the HLA‐G gene. Our results revealed higher frequency for rs17179101C (97%), rs1707T (92%) and rs9380142A (73%) alleles. Greater frequencies for the tested genotypes were observed in 3027C/C (rs17179101) (93%), 14 bp (rs1704) ins/del (92%), +3003T/T (rs1707) (85%) and +3035C/T (rs17179108) (79%) SNP genotypes. Moreover, we observed a significant association of sHLA‐G with +3010G/C (rs1710) SNP. In conclusion, we showed a significant association between 3010G/C (rs1710) SNP and the sHLA‐G level among our sample for Saudi populations. Our findings demonstrated that specific SNP within the HLA‐G gene is linked to sHLA‐G molecule secretion, suggesting sHLA‐G levels may be regulated genetically.