Rapid quantification of viable Legionella in nuclear cooling tower waters using filter cultivation, fluorescent in situ hybridization and solid-phase cytometry

Author:

Baudart J.12,Guillaume C.34,Mercier A.12,Lebaron P.12,Binet M.3

Affiliation:

1. Sorbonne Universités; UPMC Univ Paris 06; USR 3579; LBBM; Observatoire Océanologique; Banyuls/mer France

2. CNRS; USR 3579; LBBM; Observatoire Océanologique; Banyuls/mer France

3. EDF; Laboratoire National d'Hydraulique et Environnement; Chatou France

4. Euro Engineering; Énergie-Environnement; Puteaux France

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Reference50 articles.

1. AFNOR (Association Française de Normalisation) 2001 Qualité de l'eau-lignes directives pour la validation des méthodes microbiologiques

2. AFNOR (Association Française de Normalisation) 2014 Water quality - Detection and enumeration of Legionella spp. and L. pneumophila. Method by direct inoculation and after concentration by membrane filtration or centrifugation

3. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs;Altschul;Nucleic Acids Res,1997

4. Phylogenetic identification and in situ detection of individual microbial cells without cultivation;Amann;Microbiol Rev,1995

5. Rapid enumeration of active Legionella pneumophila in freshwater environments by the microcolony method combined with direct fluorescent antibody staining;Baba;Microbes Environ,2012

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