Affiliation:
1. Department of Obstetrics and Gynecology Reproductive Medicine Center Chinese PLA General Hospital Beijing China
2. Shenzhen Key Laboratory of Fertility Regulation Center of Assisted Reproduction and Embryology the University of Hong Kong – Shenzhen Hospital Shenzhen China
Abstract
AbstractProblemThe phenotypes and functions of B and CD4+ T‐helper cell subsets during chronic inflammation of the endometria remain largely unexplored. This study aimed to investigate the characteristics and functions of follicular helper T (Tfh) cells to understand the pathological mechanisms of chronic endometritis (CE).Method of studyEighty patients who underwent hysteroscopic and histopathological examinations for CE were divided into three groups—those with positive results for hysteroscopy and CD138 staining (DP), negative results for hysteroscopy but positive CD138 staining (SP), and negative results for hysteroscopy and CD138 staining (DN). The phenotypes of B cells and CD4+ T‐cell subsets were analyzed using flow cytometry.ResultsCD38+ and CD138+ cells were mainly expressed in the non‐leukocyte population of the endometria, and the endometrial CD19+CD138+ B cells were fewer than the CD3+CD138+ T cells. The percentage of Tfh cells increased with chronic inflammation in the endometria. Additionally, the elevated percentage of Tfh cells correlated with the number of miscarriages.ConclusionsCD4+ T cells, particularly Tfh cells, may be critical in chronic endometrial inflammation and affect its microenvironment, thereby regulating endometrial receptivity, compared to B cells.
Funder
Sanming Project of Medicine in Shenzhen
National Key Research and Development Program of China
Shenzhen Fundamental Research Program
Subject
Obstetrics and Gynecology,Reproductive Medicine,Immunology,Immunology and Allergy,Obstetrics and Gynecology,Immunology