Affiliation:
1. Laboratory of Genome Science Graduate School of Tokyo University of Marine Science and Technology Tokyo Japan
2. Department of Fisheries and Aquaculture, Faculty of Fisheries and Marine Sciences and Technology University of Ruhuna Matara Sri Lanka
3. Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine Mansoura University Mansoura Egypt
Abstract
AbstractVibrio nigripulchritudo causes vibriosis in penaeid shrimps. Here, we used Illumina and Nanopore sequencing technologies to sequence the genomes of three of its strains (TUMSAT‐V. nig1, TUMSAT‐V. nig2, and TUMSAT‐V. nig3) to explore opportunities for disease management. Putative virulence factors and mobile genetic elements were detected while evaluating the phylogenetic relationship of each isolated strain. The genomes consisted of two circular chromosomes (I and II) plus one or two plasmids. The size of chromosome I ranged from 4.02 to 4.07 Mb with an average GC content of 46%, while the number of predicted CDSs ranged from 3563 to 3644. The size of chromosome II ranged from 2.16 to 2.18 Mb, with an average GC content of 45.5%, and the number of predicted CDSs ranged from 1970 to 1987. Numerous virulence genes were identified related to adherence, antiphagocytosis, chemotaxis, motility, iron uptake, quorum sensing, secretion systems, and toxins in all three genomes. Higher numbers of prophages and genomic islands found in TUMSAT‐V. nig1 suggest that the strain has experienced numerous horizontal gene transfer events. The presence of antimicrobial resistance genes suggests that the strains have multidrug resistance. Comparative genomic analysis showed that all three strains belonged to the same clade.
Funder
Japan Science and Technology Agency
Japan Society for the Promotion of Science London
Subject
Veterinary (miscellaneous),Aquatic Science
Cited by
1 articles.
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