First molecular characterization of Blastocystis subtypes from domestic animals (sheep and cattle) and their animal‐keepers in Ilam, western Iran: A zoonotic concern

Author:

Shams Morteza1,Bahrami Ali Mohammad2,Mousivand Asma3,Shamsi Laya4,Asghari Ali5ORCID,Shahabi Saeed6,Sadrebazzaz Alireza7

Affiliation:

1. Zoonotic Diseases Research Center Ilam University of Medical Sciences Ilam Iran

2. Faculty of Para‐Veterinary Medicine Ilam University Ilam Iran

3. Department of Pathobiology, School of Veterinary Medicine Shiraz University Shiraz Iran

4. Department of Pathobiology, Faculty of Veterinary Medicine Urmia University Urmia Iran

5. Department of Parasitology and Mycology, School of Medicine Shiraz University of Medical Sciences Shiraz Iran

6. Department of Biology and Control of Disease Vectors, School of Health Shiraz University of Medical Sciences Shiraz Iran

7. Razi Vaccine & Serum Research Institute Agricultural Research, Education and Extension Organization Mashhad Iran

Abstract

AbstractA total of 360 fecal samples were randomly collected from 150 cattle, 150 sheep, and 60 humans (30 people with close animal contact and 30 individuals without close animal contact) at 10 farms in Ilam, western Iran from June 2022 to August 2023. All samples were directly examined for Blastocystis by zinc sulfate flotation, followed by microscopic observation. Positive samples were further subtyped using conventional PCR and sequencing methods. A mean prevalence of 5.3% (16/300) was estimated for Blastocystis infection among examined animals, with 6% and 4.7% for cattle and sheep, respectively. Among the people who had close and non‐close animal contact, 16.7% (5/30) and 3.3% (1/30) were infected with Blastocystis, respectively (p < 0.05). All 22 positive samples were successfully sequenced at the SSU rRNA locus. Accordingly, Blastocystis isolates infecting domestic animals in Ilam belonged to the four STs (ST1‐ST3, and ST10). Of the 16 animal isolates, nine sequences (four ST10, three ST3, and two ST1) were related to cattle, and seven sequences (three ST10, two ST3, and two ST2) were isolated from sheep. Among the six human isolates, ST3 was the most predominant ST, followed by STs 1, 2, 6, and 7 (one case each). Of note, ST1‐ST3 were isolated in various farms both from animals and their breeders, which indicates the possible circulation of these STs between animal and human populations.

Funder

Ilam University of Medical Sciences

Publisher

Wiley

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