Affiliation:
1. Department of Crop Physiology University of Agricultural Sciences Bengaluru India
2. Centre for Crop Systems Analysis, Department of Plant Sciences Wageningen University & Research Wageningen the Netherlands
3. Wheat Genetics Resource Center and Department of Plant Pathology Kansas State University Manhattan Kansas USA
4. Department of Pathology and Microbiology University of Nebraska Medical Centre Omaha Nebraska USA
5. Regional Centre for Biotechnology Faridabad Haryana India
Abstract
AbstractWater‐saving attempts for rice cultivation often reduce yields. Maintaining productivity under drought is possible when rice genotypes are bred with improved metabolism and spikelet fertility. Although attempts have been made to introgress water mining and water use efficiency traits, combining acquired tolerance traits (ATTs), that is, specific traits induced or upregulated to better tolerate severe stress, appears equally important. In our study, we screened 90 rice germplasm accessions that represented the molecular and phenotypic variations of 851 lines of the 3 K rice panel. Utilising phenomics, we identified markers linked to ATTs through association analysis of over 0.2 million SNPs derived from whole‐genome sequences. Propensity to respond to ‘induction’ stress varied significantly among genotypes, reflecting differences in cellular protection against oxidative stress. Among the ATTs, the hydroxyl radical and proline contents exhibited the highest variability. Furthermore, these significant variations in ATTs were strongly correlated with spikelet fertility. The 43 significant markers associated with ATTs were further validated using a different subset of contrasting genotypes. Gene expression studies and metabolomic profiling of two well‐known contrasting genotypes, APO (tolerant) and IR64 (sensitive), identified two ATT genes: AdoMetDC and Di19. Our study highlights the relevance of polyamine biosynthesis in modulating ATTs in rice. Genotypes with superior ATTs and the associated markers can be effectively employed in breeding rice varieties with sustained spikelet fertility and grain yield under drought.
Funder
Science and Engineering Research Board
Wageningen University Fund
Subject
Cell Biology,Plant Science,Genetics,General Medicine,Physiology
Cited by
2 articles.
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