Affiliation:
1. Institute of Medical Microbiology, Virology and Hygiene University Medical Center Hamburg Eppendorf Hamburg Germany
2. UKE Microscopy Imaging Facility University Medical Center Hamburg Eppendorf Hamburg Germany
Abstract
AbstractSince its introduction in 2017, MINFLUX nanoscopy has shown that it can visualise fluorescent molecules with an exceptional localisation precision of a few nanometres. In this overview, we provide a brief insight into technical implementations, fluorescent marker developments and biological studies that have been conducted in connection with MINFLUX imaging and tracking. We also formulate ideas on how MINFLUX nanoscopy and derived technologies could influence bioimaging in the future. This insight is intended as a general starting point for an audience looking for a brief overview of MINFLUX nanoscopy from theory to application.