Affiliation:
1. Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs Guangdong Provincial Key Laboratory of High Technology for Plant Protection Guangzhou China
2. College of Plant Protection South China Agricultural University Guangzhou China
3. Yuxi Branch of Yunnan Tobacco Company Engineering Center for Biological Control of Diseases and Pests in Tobacco Industry Yuxi China
Abstract
AbstractIn April 2023, leaf curl, crinkling symptoms were found on Conyza canadensis plants around tobacco fields located at Yuxi, Yunan province, China. PCR detection with genus‐specific primers showed that symptomatic C. canadensis plants was infected by begomovirus. The full‐length viral components and satellite molecules from the diseased C. canadensis (YN‐2023) were obtained by enrichment of circular DNA with rolling circle amplification, restriction enzyme digestion, cloning, and DNA sequencing. The viral component (2, 750 nt, OR509733) shared the highest nt identity at 92.4% to tobacco leaf curl Yunnan virus isolate YN5741 (TbLCYNV‐YN5741 [CN:Yn:Age:16], MN218671). A satellite molecule (1, 339 nt, OR509734) shared the highest nt identity at 86.5% to tobacco curly shoot betasatellite isolate Y115 (TbCSB‐Y115 [CN:Yn:03], AJ457822). Another satellite molecule (1, 357 nt, OR509735) shared the highest nt identity at 94.2% to ageratum yellow vein Singapore alphasatellite (AYVSGA‐[Singapore:1998], NC003414). Based on the threshold for classification of Begomovirus, Betasatellite, Alphasatellite, YN‐2023 was a new strain of TbLCYNV and associated with TbCSB and AYVSGA. To our best knowledge, this is the first report that C. canadensis is a natural host of TbLCYNV.
Funder
National Natural Science Foundation of China