Current outcomes when optimizing ‘standard’ sample preparation for single‐particle cryo‐EM

Author:

CARRAGHER B.1ORCID,CHENG Y.2ORCID,FROST A.3ORCID,GLAESER R.M.4ORCID,LANDER G.C.5ORCID,NOGALES E.678ORCID,WANG H.‐W.9

Affiliation:

1. Simon Electron Microscopy Center New York Structural Biology Center New York U.S.A.

2. HHMI and Department of Biochemistry and Biophysics University of California San Francisco California U.S.A.

3. Department of Biochemistry and Biophysics University of California San Francisco San Francisco California U.S.A.

4. Lawrence Berkeley National Laboratory University of California Berkeley California U.S.A.

5. Department of Integrative Structural and Computational Biology The Scripps Research Institute La Jolla California U.S.A.

6. Molecular and Cell Biology Department University of California Berkeley Berkeley California U.S.A.

7. MBIB Division, Lawrence Berkeley National Laboratory University of California Berkeley California U.S.A.

8. Howard Hughes Medical Institute University of California Berkeley Berkeley California U.S.A.

9. Ministry of Education Key Laboratory of Protein Sciences Beijing Advanced Innovation Center for Structural Biology Beijing Frontier Research Center of Biological Structures, School of Life Sciences Tsinghua University Beijing China

Funder

Simons Foundation

National Science Foundation

American Asthma Foundation

Amgen

Publisher

Wiley

Subject

Histology,Pathology and Forensic Medicine

Reference35 articles.

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2. ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space

3. Eliminating effects of particle adsorption to the air/water interface in single‐particle cryo‐electron microscopy: bacterial RNA polymerase and CHAPSO;Chen J.;J. Struct. Biol.,2019

4. Structural Survey of Broadly Neutralizing Antibodies Targeting the HIV-1 Env Trimer Delineates Epitope Categories and Characteristics of Recognition

5. Spotiton: New features and applications

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