Metabolomics profiling of seminal plasma in obesity‐induced asthenozoospermia

Author:

Zhou Yan‐fen1,Hou Yu‐yang1,Ban Qian1,Zhang Meng‐ling2,Huang Tao2,Ma Bo2,Shi Liang3,Zhang Qi4ORCID

Affiliation:

1. College of Biotechnology and Pharmaceutical Engineering Nanjing Tech University Nanjing China

2. College of Pharmaceutical Sciences Nanjing Tech University Nanjing China

3. Department of Urology, Affiliated Drum Tower Hospital Medical School of Nanjing University Nanjing Jiangsu China

4. College of Food Science and Light Industry Nanjing Tech University Nanjing China

Abstract

AbstractBackgroundAsthenozoospermia is one of the essential causes of male infertility, and its incidence is significantly higher in obese men. Due to its complex etiology and unknown pathomechanism, the diagnosis and treatment of obesity‐induced asthenozoospermia is a prevalent problem in reproductive medicine.ObjectiveThis study aims to explore major differential metabolites and metabolic pathways in seminal plasma and pathological mechanisms for obesity‐induced asthenozoospermia.Materials and MethodsWe performed non‐target metabolomic studies on the seminal plasma of healthy men with normal semen parameters (HN group, n = 20), obese men with normal semen parameters (ON group, n = 20), and men with obesity‐induced asthenozoospermia (OA group, n = 20) based on gas chromatography‐mass spectrometry. Metabolic profilings and related pathway analyses were conducted to discriminate differential metabolites and metabolic pathways.ResultsA total of 20 differential metabolites including fructose, succinic acid, aconitic acid, methylmaleic acid, glucopyranose, serine, valine, leucine, phenylalanine, glycine, glutamic acid, alanine, proline and threonine were identified in HN group and ON group; 24 differential metabolites including glucose, fructose, pyruvic acid, citric acid, succinic acid, aconitic acid, glucopyranose, glutamic acid, valine, leucine, glycine, phenylalanine, lysine, citrulline, proline and alanine were produced in OA group and ON group; and 28 differential metabolites including glucose, fructose, citric acid, succinic acid, glucopyranose, valine, glycine, serine, leucine, phenylalanine, alanine, threonine, proline, glutamic acid, citrulline, lysine and tyrosine were produced in OA group and HN group. In addition, abnormal energy metabolism including carbohydrate metabolism (TCA cycle, glycolysis/gluconeogenesis, and pyruvate metabolism) and amino acid metabolism (phenylalanine, tyrosine, and tryptophan biosynthesis, D‐glutamine and D‐glutamate metabolism; phenylalanine metabolism, etc.) were found in ON group and OA group.ConclusionObesity could affect the metabolite composition in seminal plasma and abnormal energy metabolism in seminal plasma mainly including carbohydrate metabolism and amino acid metabolism were closely related to obesity‐induced asthenozoospermia.

Funder

Jiangsu Provincial Key Research and Development Program

Publisher

Wiley

Subject

Urology,Endocrinology,Reproductive Medicine,Endocrinology, Diabetes and Metabolism

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