Efficient production of recombinant DNA proteins in Saccharomyces cerevisiae by controlled high‐cell‐density fermentation
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Published:1991-08
Issue:1
Volume:14
Page:82-92
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ISSN:0885-4513
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Container-title:Biotechnology and Applied Biochemistry
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language:en
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Short-container-title:Biotech and App Biochem
Author:
Alberghina L.,Porro D.,Martegani E.,Ranzi BM
Abstract
High levels of expression of heterologous proteins (from 5 to 15% of total cell proteins) in the budding yeast Saccharomyces cerevisiae have been obtained previously by the use of the inducible strong hybrid promoter UASGAL/CYC1, in batch as well in continuous cultures. However, in order to maximize the yield of heterologous proteins, a computer controlled fed‐batch fermentation is essential. For this reason we have developed a fed‐batch system based on a semiconductor gas detector that measures ethanol in the outflow gases. The optimal conditions are described for very high production (up to 1550 mg/liter), with both high productivity (up to 100–120 mg/liter/h) and high yield (up to 15 mg of protein/g of dry biomass), of heterologous protein driven by the UASGAL/CYC1 promoter in a completely computer controlled fed‐batch fermentation of budding yeast. However, high production was dependent upon the addition of a large amount of galactose. The process was improved by developing a new, more easily inducible, vector system obtained by subcloning the GAL4 gene.
Subject
Process Chemistry and Technology,Drug Discovery,Applied Microbiology and Biotechnology,Biomedical Engineering,Molecular Medicine,General Medicine,Bioengineering,Biotechnology
Cited by
1 articles.
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1. Production of Microbial Biomass;Biotechnology Set;2001-05-10