Affiliation:
1. Department of Obstetrics and Gynecology Minhang Hospital Fudan University Shanghai China
2. Department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai China
Abstract
AbstractBackgroundValue analysis of a small‐molecule fluorescent probe for methylation detection in different cervical lesions.Materials and Methods(1) The grayscale values of distinct lesion tissues were remarkably distinct among the four groups (p < 0.05). The comparison of the grayscale value between the two groups showed that the CA group noticeably exceeded the LSIL and cervicitis groups, and the HSIL group was apparently higher than the LSIL and cervicitis groups (p < 0.05); (2) The mean grayscale values of the enrolled subjects were calculated with 55.21 as the midline, with >55.21 as positive and ≤55.21 as negative.ResultsThe results showed that the positive rate of the cervicitis group was 0.00%, the LSIL group 67.74%, the HSIL group 83.33%, and the CA group 100.00%. The results among the four groups were notably distinct (p < 0.05); (3) The comparison among DAPI, probe, bright, and merged images of cervicitis, LSIL, HSIL, and CA indicated that different cervical lesions were with quite various stains.ConclusionThe grayscale value, positive rate, and stained picture of distinct cervical lesions were remarkably different. The small‐molecule fluorescent probe has a good value in differentiating cervical lesions and can be considered for popularization and application.