Simultaneous mutations in ITPK4 and MRP5 genes result in a low phytic acid level without compromising salt tolerance in Arabidopsis

Author:

Ren Yuying12ORCID,Jiang Mengdan3ORCID,Zhu Jian‐Kang45ORCID,Zhou Wenkun3ORCID,Zhao Chunzhao1ORCID

Affiliation:

1. Key Laboratory of Plant Design, National Key Laboratory of Plant Molecular Genetics, Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences the Chinese Academy of Sciences Shanghai 200032 China

2. University of Chinese Academy of Sciences Beijing 100190 China

3. State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences China Agricultural University Beijing 100193 China

4. Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and Technology Shenzhen 518055 China

5. Ministry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies, Nanfan Research Institute Chinese Academy of Agricultural Sciences Sanya 572024 China

Abstract

ABSTRACTGeneration of crops with low phytic acid (myo‐inositol‐1,2,3,4,5,6‐hexakisphosphate (InsP6)) is an important breeding direction, but such plants often display less desirable agronomic traits. In this study, through ethyl methanesulfonate‐mediated mutagenesis, we found that inositol 1,3,4‐trisphosphate 5/6‐kinase 4 (ITPK4), which is essential for producing InsP6, is a critical regulator of salt tolerance in Arabidopsis. Loss of function of ITPK4 gene leads to reduced root elongation under salt stress, which is primarily because of decreased root meristem length and reduced meristematic cell number. The itpk4 mutation also results in increased root hair density and increased accumulation of reactive oxygen species during salt exposure. RNA sequencing assay reveals that several auxin‐responsive genes are down‐regulated in the itpk4‐1 mutant compared to the wild‐type. Consistently, the itpk4‐1 mutant exhibits a reduced auxin level in the root tip and displays compromised gravity response, indicating that ITPK4 is involved in the regulation of the auxin signaling pathway. Through suppressor screening, it was found that mutation of Multidrug Resistance Protein 5 (MRP5)5 gene, which encodes an ATP‐binding cassette (ABC) transporter required for transporting InsP6 from the cytoplasm into the vacuole, fully rescues the salt hypersensitivity of the itpk4‐1 mutant, but in the itpk4‐1 mrp5 double mutant, InsP6 remains at a very low level. These results imply that InsP6 homeostasis rather than its overall amount is beneficial for stress tolerance in plants. Collectively, this study uncovers a pair of gene mutations that confer low InsP6 content without impacting stress tolerance, which offers a new strategy for creating “low‐phytate” crops.

Funder

National Natural Science Foundation of China

Science and Technology Commission of Shanghai Municipality

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3