Magnaporthe oryzae endoplasmic reticulum membrane complex regulates the biogenesis of membrane proteins for pathogenicity

Author:

Liu Ning12ORCID,Huang Manna1ORCID,Liang Xinyuan1,Cao Miao1,Lun Zhiqin1,Zhang Yan1,Yang Jun1ORCID,Bhadauria Vijai1,Zhao Wensheng1ORCID,Yan Jiye2,Peng You‐Liang1ORCID,Lu Xunli1ORCID

Affiliation:

1. State Key Laboratory of Agrobiotechnology and MOA Key Laboratory of Pest Monitoring and Green Management China Agricultural University Beijing 100193 China

2. Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences Beijing 100097 China

Abstract

Summary In eukaryotes, the majority of newly synthesized integral membrane proteins are inserted into the endoplasmic reticulum (ER) membrane before transferred to their functional sites. The conserved ER membrane complex (EMC) takes part in the insertion process for tail‐anchored membrane proteins. However, the function of EMC in phytopathogenic fungi has not been characterized. Here, we report the identification and functional characterization of two EMC subunits MoEmc5 and MoEmc2 in Magnaporthe oryzae. The knockout mutants ΔMoemc5 and ΔMoemc2 exhibit substantial defect in autophagy, pathogenicity, cell wall integrity, and magnesium ion sensitivity. We demonstrate that the autophagy process was severely impaired in the ΔMoemc5 and ΔMoemc2 mutants because of the low‐protein steady‐state level of Atg9, the sole membrane‐associated autophagy protein. Furthermore, the protein level of membrane proteins Chs4, Fks1, and MoMnr2 is also significantly reduced in the ΔMoemc5 and ΔMoemc2 strains, leading to their supersensitivity to Calcofluor white, Congo red, and magnesium. In addition, MoEmc5, but not MoEmc2, acts as a magnesium transporter independent of its EMC function. Magnaporthe oryzae EMC regulates the biogenesis of membrane proteins for autophagy and virulence; therefore, EMC subunits could be potential targets for fungicide design in the future.

Funder

China Agricultural Research System

China Postdoctoral Science Foundation

National Natural Science Foundation of China

Publisher

Wiley

Subject

Plant Science,Physiology

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