Tissue-print and squash real-time PCR for direct detection of ‘CandidatusLiberibacter’ species in citrus plants and psyllid vectors

Author:

Bertolini E.1,Felipe R. T. A.2,Sauer A. V.3,Lopes S. A.4,Arilla A.1,Vidal E.1,Mourão Filho F. A. A.2,Nunes W. M. C.3,Bové J. M.5,López M. M.1,Cambra M.1

Affiliation:

1. Plant Protection Centre; Instituto Valenciano de Investigaciones Agrarias (IVIA); 46113 Moncada Valencia Spain

2. Escola Superior de Agricultura ‘Luiz de Queiroz’ (ESALQ); Universidade de São Paulo (USP); 13418-900 Piracicaba São Paulo Brazil

3. Nucleo de Pesquisa em Biotecnologia Aplicada; Centro de Ciências Agrárias (CCA); Universidade Estadual de Maringá (UEM); 87020-900 Maringá Paraná Brazil

4. Fundecitrus; 14807-040 Araraquara São Paulo Brazil

5. Institut National de la Recherche Agronomique (INRA); Université Ségalen Bordeaux II; 33140 Villenave d’Ornon France

Funder

CE-EU FP7-ERANET EUPHRESCO

Spanish Ministry of Agriculture, Food and Environment (MAGRAMA)

Spanish National Institute of Agricultural Research (INIA)

Publisher

Wiley

Subject

Horticulture,Plant Science,Genetics,Agronomy and Crop Science

Reference47 articles.

1. Base científica para a erradicação de plantas sintomáticas e assintomáticas de Huanglongbing (HLB, Greening) visando o controle efetivo da doença;Belasque;Tropical Plant Pathology,2009

2. Lessons from huanglongbing management in São Paulo State, Brazil;Belasque;Journal of Plant Pathology,2010

3. Quantitative detection of Citrus tristeza virus in plant tissues and single aphids by real-time RT-PCR;Bertolini;European Journal of Plant Pathology,2008

4. Bertolini E Cambra M Serra P et al 2010 Direct procedure for specific detection of ‘Ca. Liberibacter’ spp. by immobilized targets and real-time PCR and kit for its detection http://www.oepm.es/pdf/ES/0000/000/02/38/61/ES-2386131_A1.pdf

5. Huanglongbing: a destructive, newly-emerging, century-old disease of citrus;Bové;Journal of Plant Pathology,2006

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