Consequences of the deletion of the major specialized metabolite biosynthetic pathways of Streptomyces coelicolor on the metabolome and lipidome of this strain

Author:

Lejeune Clara1,Abreu Sonia2,Guérard Florence3,Askora Ahmed14,David Michelle1,Chaminade Pierre2,Gakière Bertrand3,Virolle Marie‐Joelle1ORCID

Affiliation:

1. Institut de Biologie Intégrative de la Cellule (I2BC, UMR 9198), Université Paris‐Saclay, CEA, CNRS, Group MES (Métabolisme Energétique Des Streptomyces) Gif‐sur‐Yvette France

2. UFR Pharmacie, Université Paris‐Saclay, CNRS, Group «Lipides, Systèmes Analytiques et Biologiques (Lip(Sys)2» Orsay France

3. Institut Des Sciences Des Plantes (IPS2, UMR 9213), Université Paris‐Saclay, CNRS, Plateforme «SPOmics‐Métabolome» Gif‐sur‐Yvette France

4. Department of Botany and Microbiology, Faculty of Science Zagazig University Zagazig Egypt

Abstract

AbstractChassis strains, derived from Streptomyces coelicolor M145, deleted for one or more of its four main specialized metabolites biosynthetic pathways (CPK, CDA, RED and ACT), in various combinations, were constructed for the heterologous expression of specialized metabolites biosynthetic pathways of various types and origins. To determine consequences of these deletions on the metabolism of the deleted strains comparative lipidomic and metabolomic analyses of these strains and of the original strain were carried out. These studies unexpectedly revealed that the deletion of the peptidic clusters, RED and/or CDA, in a strain deleted for the ACT cluster, resulted into a great increase in the triacylglycerol (TAG) content, whereas the deletion of polyketide clusters, ACT and CPK had no impact on TAG content. Low or high TAG content of the deleted strains was correlated with abundance or paucity in amino acids, respectively, reflecting high or low activity of oxidative metabolism. Hypotheses based on what is known on the bio‐activity and the nature of the precursors of these specialized metabolites are proposed to explain the unexpected consequences of the deletion of these pathways on the metabolism of the bacteria and on the efficiency of the deleted strains as chassis strains.

Funder

Agence de l'innovation de Défense

Publisher

Wiley

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