Affiliation:
1. Plant Research Department Nuclear Research Center, Egyptian Atomic Energy Authority Cairo Egypt
2. Radiation Protection and Safety Department, Hot Labs Center Egyptian Atomic Energy Authority Cairo Egypt
Abstract
Abstract
Aims
This study aims at immobilization of fungal mutants on nanosilica (NSi)-carriers for designing efficient biosorbents as a significant new technology for decontamination practices and maximizing their heavy metal (HM) sorption proficiency through the experimental design methodology.
Materials and Results
Endophytic fungal mutant strains, Chaetomium globosum El26 mutant and Alternaria alternata S5 mutant were heat inactivated and then immobilized, each separately, on NSi carriers to formulate two separated nano-biosorbents. The formulated NSi-Chaetomium globosum El26 mutant (NSi-Chae El26 m) was investigated for Pb+2 uptake while, the formulated NSi-Alternaria alternata S5 mutant (NSi-Alt S5 m) was investigated for Cd+2 uptake, each through a batch equilibrium protocol. Before and after the metal sorption process, the designed nano-biosorbents were characterized via scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Fourier transform infrared analysis. Sorption pH, contact time, sorbent concentration, and initial HM concentration were statistically optimized using a Box–Behnken design. Results showed that NSi-Chae El26 m was efficient in Pb+2 uptake with maximum biosorption capacities of 199.0, while NSi-Alt S5 m was efficient in Cd+2 uptake with maximum biosorption capacities of 162.0 mg g−1. Moreover, the equilibrium data indicated that the adsorption of Pb+2 and Cd+2 by the tested nano-biosorbents fitted to the Freundlich isotherm.
Conclusions
The formulated nano-biosorbents resulted in higher HM biosorption of metal ions from aqueous solution than that obtained by the free fungal biomass. The biosorption statistical modelling described the interactions between the tested sorption parameters and predicted the optimum values for maximum HM biosorption capacity by the two designed nano-biosorbents.
Significance and Impact of The Study
These findings verify that members of the endophytic fungal genera Alternaria and Chaetomium are suitable to produce nano-biosorbents for decontamination practices after treatment by gamma mutagenesis, heat inactivation, and NSi immobilization. Moreover, statistical optimization can assist to evaluate the optimal conditions to produce such bioremediation material.
Publisher
Oxford University Press (OUP)
Subject
Applied Microbiology and Biotechnology,General Medicine,Biotechnology
Reference38 articles.
1. Response surface methodology for cadmium biosorption on Pseudomonas aeruginosa;Ahmady-Asbchin;Water Science and Technology,2016
2. Application of response surface methodology for optimization of lead biosorption in an aqueous solution by Aspergillus niger;Amini;Journal of Hazardous Materials,2008
3. Recent trends in nanomaterials applications in environmental monitoring and remediation;Das;Environmental Science and Pollution Research,2015
4. Unlocking the biosynthetic potential of Penicillium roqueforti for hyper-production of the immunosuppressant mycophenolic acid: gamma radiation mutagenesis and response surface optimization of fermentation medium;El-Sayed;Biotechnology and Applied Biochemistry,2022
5. Production of the anticancer drug taxol by the endophytic fungus Epicoccum nigrum TXB502: enhanced production by gamma irradiation mutagenesis and immobilization technique;El-Sayed;Applied Microbiology and Biotechnology,2020
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献