IGJ depletion suppresses proliferation, inflammation, and motility of rheumatoid arthritis fibroblast‐like synoviocytes via targeting NF‐κB pathway

Abstract

AbstractObjectiveTo investigate the impact of IGJ on the proliferation, inflammation, and motility of rheumatoid arthritis (RA) fibroblast‐like synoviocytes and elucidate the underlying mechanism.MethodsThe expression of IGJ RA fibroblast‐like synoviocytes was assessed using immunoblot and qPCR. Cell growth was evaluated using CCK‐8 and FCM assays. The effects on inflammatory response were determined by ELISA and immunoblot assays. Cell motility was assessed using transwell and immunoblot assays. The mechanism was further confirmed using immunoblot assays.ResultsIGJ expression was found to be elevated in fibroid synovial cells of RA. IGJ ablation inhibited the growth of MH7A cells and suppressed the inflammatory response. Knockdown of IGJ also blocked cell motility. Mechanically, the knockdown of IGJ suppressed the NF‐κB axis in MH7A cells.ConclusionIGJ suppresses RA in fibroblast‐like synoviocytes via NF‐κB pathway.