Kidney cell culture Macaca fascicularis as a candidate for vaccine development and in vitro model

Abstract

AbstractBackgroundCell culture is the proliferation of a cell population in vitro by isolating from the original tissue or growing from existing ones. One essential source is the monkey kidney cell cultures which have an essential role in biomedical study. This is due to the significant homology between the human and macaque genomes making these useful for cultivating human viruses, especially enteroviruses, and growing vaccines.MethodsThis study developed cell cultures derived from the kidney of Macaca fascicularis (Mf) and validated its gene expression.ResultsThe primary cultures were successfully subcultured up to six passages, grew as monolayers, and exhibited epithelial‐like morphology. The cultured cells remained heterogeneous in phenotype and they expressed CD155 and CD46 as viral receptors, cell morphology (CD24, endosialin, and vWF), proliferation, also apoptosis markers (Ki67 and p53).ConclusionsThese results indicated that the cell cultures can be used as in vitro model cells for vaccine development and bioactive compound.