A commercial SnF2 toothpaste formulation reduces simulated human plaque biofilm in a dynamic typodont model

Author:

Khosravi Yalda1,Palmer Sara2,Daep Carlo A.3,Sambanthamoorthy Karthik3,Kumar Purnima2,Dusane Devendra H.1,Stoodley Paul145ORCID

Affiliation:

1. Department of Microbial Infection and Immunity Ohio State University Columbus Ohio USA

2. College of Dentistry The Ohio State University Columbus Ohio USA

3. Colgate-Palmolive company Piscataway New Jersey USA

4. Department of Orthopaedics Ohio State University Columbus Ohio USA

5. National Centre for Advanced Tribology (nCATS) and National Biofilm Innovation Centre (NBIC), Mechanical Engineering University of Southampton Southampton UK

Abstract

Abstract Aims We present a dynamic typodont biofilm model (DTBM) incorporating (1) human dentition anatomy, (2) fluid flow over intermittently fluid bathed tooth surfaces and (3) an oxic headspace to allow aerobic and anaerobic niches to develop naturally, as a screening tool to assess the effect of stannous fluoride (SnF2) toothpaste against a simulated human plaque biofilm (SPB). Methods and results First, hydroxyapatite (HA) coupons were inoculated with human saliva/plaque and cultured at 37°C under air. Selected species representative of common commensal and anaerobic pathogens were quantified for relative abundance changes over 4 days by PCR densitometry to confirm the culture conditions allowed the proliferation of these species. A continuous culture DTBM reactor on a rocker table was inoculated with saliva/plaque and incubated at 37°C for 24 h. Tooth shear stress was estimated by particle tracking. A SnF2 toothpaste solution, or a sham rise was administered twice daily for 3 days to mimic routine oral hygiene. SPB biomass was assessed by total bacterial DNA and methylene blue (MB) staining. Early colonizer aerobes and late colonizer anaerobes species were detected in the HA and DTBM, and the trends in changing abundance were consistent with those seen clinically. Conclusions Treatment with the SnF2 solution showed significant reductions of 53.05% and 54.4% in the SPB by MB staining and DNA, respectively. Significance and impact of study: The model has potential for assessing dentition anatomy and fluid flow on the efficacy of antimicrobial efficacy against localized SPB and may be amenable to the plaque index clinical evaluation.

Funder

Colgate-Palmolive Company

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Reference33 articles.

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4. Stannous fluoride effects on enamel: a systematic review;Fiorillo;Biomimetics,2020

5. A simplified system for simulation of Streptococcus mutans biofilm on healthy extracted human tooth as dental plaque model;Halib;Journal of Applied Pharmaceutical Science,2019

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