Natural occurrence of cucumber mosaic virus infecting Adenium obesum plants in Brazil

Author:

Bello Vinicius Henrique1ORCID,Carpim Larissa1,Yuki Valdir Atsushi2,Beserra Júnior José Evando Aguiar3ORCID,Nagata Tatsuya4,Rezende Jorge Alberto Marques1ORCID,Kitajima Elliot Watanabe1

Affiliation:

1. Departamento de Fitopatologia e Nematologia Universidade de São Paulo/Esalq Piracicaba Brazil

2. Centro de Fitossanidade Instituto Agronômico de Campinas Campinas Brazil

3. Departamento de Fitotecnia Universidade Federal do Piauí Teresina Piauí Brazil

4. Departamento de Biologia Molecular Universidade de Brasília Brasília Federal District Brazil

Abstract

AbstractIn 2022, virus‐like symptoms were observed in Adenium obesum plants in Brazil. High‐throughput sequencing analysis found three contig sequences of cucumber mosaic virus (CMV) genome segments, which were of 3352, 3006 and 2221 nucleotides, and shared 98.59%, 95.02% and 98.05% nucleotide identities with the nucleotide sequences of CMV RNAs 1, 2 and 3, respectively. Phylogenetic analysis of the coat protein gene indicated that the CMV isolate belongs to the subgroup IB. Symptoms of mottle like those observed in the field samples were reproduced on mechanically inoculated plants of A. obesum, and RT‐PCR confirmed the CMV infection. CMV was transmitted by Aphis gossypii to A. obesum plants. This is the first report of CMV infecting A. obesum plants in Brazil.

Funder

Fundação de Amparo à Pesquisa do Estado de São Paulo

Publisher

Wiley

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