Auxin response factors fine‐tune lignin biosynthesis in response to mechanical bending in bamboo

Author:

Wang Wenjia1ORCID,Li Yigang1ORCID,Cai Changyang1ORCID,Zhu Qiang1ORCID

Affiliation:

1. Basic Forestry and Proteomics Center (BFPC), College of Forestry, Haixia Institute for Science and Technology Fujian Agriculture and Forestry University 350002 Fujian China

Abstract

Summary Lignin contributes to plant mechanical properties during bending loads. Meanwhile, phytohormone auxin controls various plant biological processes. However, the mechanism of auxin's role in bending‐induced lignin biosynthesis was unclear, especially in bamboo, celebrated for its excellent deformation stability. Here, we reported that auxin response factors (ARF) 3 and ARF6 from Moso bamboo (Phyllostachys edulis (Carrière) J. Houz) directly regulate lignin biosynthesis pathway genes, and affect lignin biosynthesis in bamboo. Auxin and lignin exhibited asymmetric distribution patterns, and auxin promoted lignin biosynthesis in response to bending loads in bamboo. Employing transcriptomic and weighted gene co‐expression network analysis approach, we discovered that expression patterns of ARF3 and ARF6 strongly correlated with lignin biosynthesis genes. ARF3 and ARF6 directly bind to the promoter regions of 4‐coumarate: coenzyme A ligase (4CL3, 4CL7, and 4CL9) or caffeoyl‐CoA O‐methyltransferase (CCoAOMT2) genes, pivotal to lignin biosynthesis, and activate their expressions. Notably, the efficacy of this binding hinges on auxin levels. Alternation of the expressions of ARF3 and ARF6 substantially altered lignin accumulation in transgenic bamboo. Collectively, our study shed light on bamboo lignification genetics. Auxin signaling could directly modulate lignin biosynthesis genes to impact plant lignin content.

Funder

Forestry Department of Fujian Province

Fujian Agriculture and Forestry University

National Key Research and Development Program of China

National Natural Science Foundation of China

Natural Science Foundation of Fujian Province

Publisher

Wiley

Subject

Plant Science,Physiology

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