Densovirus infection facilitates plant–virus transmission by an aphid

Author:

Dong Yonghao1ORCID,Li Tong2ORCID,Hou Yuanyuan1ORCID,Wilson Kenneth3ORCID,Wang Xiufang1ORCID,Su Chenyu1ORCID,Li Yunhe4ORCID,Ren Guangwei1ORCID,Xu Pengjun1ORCID

Affiliation:

1. Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management Tobacco Research Institute of Chinese Academy of Agricultural Sciences Qingdao 266101 China

2. Institute of Plant Protection Henan Academy of Agricultural Sciences Zhengzhou 450002 China

3. Lancaster Environment Centre Lancaster University Lancaster LA1 4YW UK

4. State Key Laboratory of Cotton Bio‐breeding and Integrated Utilization, School of Life Sciences and College of Agriculture Henan University Kaifeng 475004 China

Abstract

Summary The interactions among plant viruses, insect vectors, and host plants have been well studied; however, the roles of insect viruses in this system have largely been neglected. We investigated the effects of MpnDV infection on aphid and PVY transmission using bioassays, RNA interference (RNAi), and GC‐MS methods and green peach aphid (Myzus persicae (Sulzer)), potato virus Y (PVY), and densovirus (Myzus persicae nicotianae densovirus, MpnDV) as model systems. MpnDV increased the activities of its host, promoting population dispersal and leading to significant proliferation in tobacco plants by significantly enhancing the titer of the sesquiterpene (E)‐β‐farnesene (EβF) via up‐regulation of expression levels of the MpFPPS1 gene. The proliferation and dispersal of MpnDV‐positive individuals were faster than that of MpnDV‐negative individuals in PVY‐infected tobacco plants, which promoted the transmission of PVY. These results combined showed that an insect virus may facilitate the transmission of a plant virus by enhancing the locomotor activity and population proliferation of insect vectors. These findings provide novel opportunities for controlling insect vectors and plant viruses, which can be used in the development of novel management strategies.

Funder

Agricultural Science and Technology Innovation Program

Publisher

Wiley

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