Affiliation:
1. National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes Tainan Taiwan
2. Division of Infectious Diseases, Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine National Cheng Kung University Tainan Taiwan
Abstract
AbstractBackgroundEpidemiological knowledge is important to guide antifungal therapy.ObjectiveThis multicentre study aimed to investigate the species distribution and antifungal susceptibility of Aspergillus isolates in Taiwan.MethodFour hundred and ninety‐two clinical Aspergillus isolates, collected during 2016–2020, were identified by calmodulin sequencing and tested for antifungal susceptibility using CLSI M38‐A3. The Cyp51A sequences of azole‐resistant Aspergillus fumigatus and Aspergillus flavus isolates were analysed.ResultsThis collection comprised 30 species from eight Aspergillus sections—Flavi (33.5%), Nigri (26.0%), Fumigati (24.2%), Terrei (10.0%), Nidulantes (5.1%), Circumdati (0.8%), Restricti (0.2%) and Aspergillus (0.2%). Sections Fumigati, Flavi and Terrei were primarily represented by A. fumigatus (99.2%), A. flavus (95.8%) and A. terreus (100%), respectively. Section Nigri comprised nine species, mostly A. welwitschiae (60.2%), A. niger (12.5%), A. brunneoviolaceus (10.9%) and A. tubingensis (10.2%). A. fumigatus (39.6%) and A. flavus (26.4%) predominated among 53 isolates from lower respiratory samples, whereas section Nigri species (46.2%) and A. terreus (29.2%) predominated among 65 isolates from ear samples. Reduced susceptibility to amphotericin B (minimal inhibitory concentration (MIC) > 1 μg/mL) was noted in A. flavus (7.0%), A. terreus (6.1%), A. nidulans and section Circumdati (A. flocculosus, A. subramanianii and A. westerdijkiae) isolates. Acquired azole resistance was observed in seven A. fumigatus (5.9%), all of which carried TR34/L98H or TR34/L98H/S297T/F495I mutation, and three A. flavus (1.9%), one of which carried G441S mutation. Reduced susceptibility to itraconazole (MIC >1 μg/mL) was noted in 55.5% of section Nigri isolates, mainly in A. welwitschiae, A. niger and A. tubingensis, whereas A. brunneoviolaceus, A. aculeatinus and A. japonicus were hypersusceptible to azoles. Anidulafungin was active against all isolates except for one isolate.ConclusionsThis study depicted the molecular epidemiology and species‐specific characteristics of Aspergillus in Taiwan, which aids in appropriate antifungal therapy and underlines the need of speciation and susceptibility testing of disease‐causing Aspergillus.
Funder
National Health Research Institutes
Subject
Infectious Diseases,Dermatology,General Medicine