Antibacterial and anti‐biofilm properties of cinnamaldehyde‐loaded nanoliposomes against Listeria monocytogenes and Salmonella enteritidis adhered to stainless steel

Abstract

SummaryThis study aimed to investigate the effect of cinnamaldehyde (CIN)‐loaded nanoliposomes (CIN‐NLs) on pre‐established biofilms of L. monocytogenes and S. enteritidis. The MIC and MBC values of CIN were 1.25 and 5 μg mL−1 against L. monocytogenes, respectively; while for S. enteritidis, MIC of 0.625 and MBC of 2.5 μg mL−1 were obtained. The CIN‐NLs displayed particle size in the range of 130.5–414.2 nm, polydispersity index (PDI) values in the range of 0.28–0.59, and ζ‐potential values above −30 mV. The encapsulation efficiency of CIN in NLs ranged from 7.47% to 27.42%. Transmission electron microscopy (TEM) displayed smooth spherical particles, and Fourier transform infrared spectroscopy (FT‐IR) results showed the successful loading of the CIN into NLs. The anti‐biofilm assays confirmed that the CIN‐NLs more efficiently prevented the formation of biofilm and extends the action time than that of free CIN. Therefore, NLs could improve the stability and long‐lasting antibacterial effect of CIN.