Identification of new amoebae strains in rainbow trout (Oncorhynchus mykiss, Walbaum) farms affected by nodular gill disease (NGD) in Northeastern Italy

Author:

Brocca Ginevra12ORCID,Truant Alessandro3,Peckova Hana4,Lisnerová Martina4,Perolo Alberto5ORCID,Fioravanti Marialetizia6,Fiala Ivan4,Gabai Gianfranco1,Quaglio Francesco1,Gustinelli Andrea6ORCID

Affiliation:

1. Department of Comparative Biomedicine and Food Science (BCA) University of Padova Legnaro Padua Italy

2. Aquatic Diagnostic Services, Atlantic Veterinary College University of Prince Edward Island Charlottetown Prince Edward Island Canada

3. Department of Animal Medicine, Production and Health University of Padova Legnaro Padua Italy

4. Institute of Parasitology, Biology Centre CAS Ceske Budejovice Czech Republic

5. Servizio Tecnico Commerciale Aquafeed, Gruppo Veronesi Verona Veneto Region Italy

6. Department of Veterinary Medical Sciences Alma Mater Studiorum University of Bologna Ozzano dell'Emilia Bologna Italy

Abstract

AbstractNodular gill disease (NGD) is an emerging condition associated with amoeba trophozoites in freshwater salmonid farms. However, unambiguous identification of the pathogens still must be achieved. This study aimed to identify the amoeba species involved in periodic NGD outbreaks in two rainbow trout (Oncorhynchus mykiss) farms in Northeastern Italy. During four episodes (February–April 2023), 88 fish were euthanized, and their gills were evaluated by macroscopic, microscopic and histopathological examination. The macroscopic and microscopic severity of the lesions and the degree of amoebae infestation were scored and statistically evaluated. One gill arch from each animal was put on non‐nutrient agar (NNA) Petri dishes for amoeba isolation, cultivation and subsequent identification with SSU rDNA sequencing. Histopathology confirmed moderate to severe lesions consistent with NGD and mild to moderate amoeba infestation. The presence of amoebae was significantly correlated with lesion severity. Light microscopy of cultured amoebae strains and SSU rDNA analysis revealed the presence of a previously characterized amoeba Naegleria sp. strain GERK and several new strains: two strains from Hartmannelidae, three vannelid amoebae from the genus Ripella and cercozoan amoeba Rosculus. Despite the uncertainty in NGD etiopathogenesis and amoebae pathogenic role, identifying known and new amoebae leans towards a possible multi‐aetiological origin.

Funder

Grantová Agentura České Republiky

Publisher

Wiley

Reference42 articles.

1. Study on rainbow trout nodular gill disease detected in Poland;Antychowicz J.;Bulletin of the Veterinary Institute in Pulawy,2007

2. Phylogenetic Relationships among Eight Eimeria Species Infecting Domestic Fowl Inferred Using Complete Small Subunit Ribosomal DNA Sequences

3. Bermúdez R. Losada A. De Azevedo A. M. Castrillo P. A. Ronza P. Rubiolo J. A. Sotelo M. &Quiroga M. I.(2019).Nodular gill disease in rainbow trout an emerging disease? 19th International Conference on Diseases of Fish and Shellfish. Abstract Book 147.

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3