Affiliation:
1. College of Life Science Zhejiang Chinese Medical University Hangzhou China
2. The Fourth School of Clinical Medicine Zhejiang Chinese Medical University Hangzhou China
3. The First Affiliated Hospital of Zhejiang Chinese Medical University Hangzhou China
Abstract
AbstractBackgroundUltraviolet (UV) exposure‐stimulated reactive oxygen species (ROS) formation in keratinocytes is a crucial factor in skin aging. Phytochemicals have become widely popular for protecting the skin from UV‐induced cell injury. Sesamin (SSM) has been shown to play a role in extensive pharmacological activity and exhibit photoprotective effects.AimTo assess the protective effect of SSM on UVA‐irradiated keratinocytes and determine its potential antiphotoaging effect.MethodsHaCaT keratinocytes pretreated with SSM were exposed to UVA radiation at 8 J/cm2 for 10 min. Cell viability and oxidative stress indicators were evaluated using a cell counting kit‐8 and lactate dehydrogenase (LDH), malondialdehyde (MDA), glutathione (GSH), and superoxide dismutase (SOD) assay kits. Apoptosis and intracellular ROS levels were analyzed using annexin V‐fluorescein isothiocyanate/propyridine iodide and dichlorodihydrofluorescein diacetate staining, respectively. Protein levels of matrix metalloprotein‐1 (MMP‐1), MMP‐9, Bax/Bcl‐2, and mitogen‐activated protein kinase (MAPK) pathway proteins, phospho‐apoptosis signal‐regulating kinase‐1 (p‐ASK‐1)/ASK‐1, phospho‐c‐Jun N‐terminal protein kinase (p‐JNK)/JNK, and p‐p38/p38 were determined using western blotting.ResultsSesamin showed no cytotoxicity until 160 μmol/L on human keratinocytes. Sesamin pretreatment (20 and 40 μM) reversed the suppressed cell viability, increased LDH release and MDA content, decreased cellular antioxidants GSH and SOD, and elevated intracellular ROS levels, which were induced by UVA irradiation. Additionally, SSM inhibited the expression of Bax, MMP‐1, and MMP‐9 and stimulated Bcl‐2 expression. In terms of the regulatory mechanisms, we demonstrated that SSM inhibits the phosphorylation of ASK‐1, JNK, and p38.ConclusionThe results suggest that SSM attenuates UVA‐induced keratinocyte injury by inhibiting the ASK‐1‐JNK/p38 MAPK pathways.