Hydrogen peroxide regulates the Osa‐miR156‐OsSPL2/OsTIFY11b module in rice

Author:

Tseng Bo‐Shun1,Huang Cheng‐Chung1,King Yu‐Chi1,Wu Ming‐Tsung123,Hsieh Chih‐Hung1,Hsieh Kun‐Ting4,Hsing Yue‐Ie2,Jeng Shih‐Tong1ORCID

Affiliation:

1. Institute of Plant Biology and Department of Life Science National Taiwan University Taipei Taiwan

2. Institute of Plant and Microbial Biology Academia Sinica Taipei Taiwan

3. Department of Civil and Environmental Engineering, South Kensington Campus Imperial College London London UK

4. Institute of Molecular Biology National Chung Hsing University Taichung Taiwan

Abstract

AbstractField‐grown rice (Oryza sativa L.), when exposed to various environmental stresses, produces high amounts of reactive oxygen species, such as H2O2. MicroRNAs (miRNAs) play crucial roles in plant stress responses. This study characterized the functions of H2O2‐regulated miRNAs in rice. Small RNA deep sequencing revealed that miR156 levels decreased following H2O2 treatment. Searches of the rice transcriptome and degradome databases indicated that OsSPL2 and OsTIFY11b are miR156‐target genes. Interactions between miR156 and OsSPL2 and OsTIFY11b were confirmed using transient expression assays through agroinfiltration. In addition, the levels of OsSPL2 and OsTIFY11b transcripts were lower in transgenic rice plants overexpressing miR156 than in wild‐type plants. The OsSPL2‐GFP and OsTIFY11b‐GFP proteins were localized to the nucleus. Yeast two‐hybrid and bimolecular fluorescence complementation assays indicated interactions between OsSPL2 and OsTIFY11b. Furthermore, OsTIFY11b interacted with OsMYC2 to regulate the expression of OsRBBI3‐3, which encodes a proteinase inhibitor. The results suggested that H2O2 accumulation in rice suppresses the expression of miR156, and induces the expression of its target genes, OsSPL2 and OsTIFY11b, whose proteins interact in the nucleus to regulate the expression of OsRBBI3‐3, which is involved in plant defense.

Funder

National Taiwan University

Publisher

Wiley

Subject

Plant Science,Physiology

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3