Zinc oxide nanoparticles functionalized with curcumin supplementation during in vitro embryo culture impaired in a concentration‐dependent‐manner blastocyst production in cattle-Reference-Cited by-同舟云学术

Zinc oxide nanoparticles functionalized with curcumin supplementation during in vitro embryo culture impaired in a concentration‐dependent‐manner blastocyst production in cattle

Published:2023-07-05 Issue:8 Volume:58 Page:1172-1175
ISSN:0936-6768
Container-title:Reproduction in Domestic Animals
language:en
Short-container-title:Reprod Domestic Animals

Author:

Miglio Luisa¹,Novaes Marco Aurélio Schiavo²,de Lima Laritza Ferreira¹,Arantes Tatiane Moraes³,Alves Kele Amaral⁴,Dias Francisca Elda Ferreira⁵,Beletti Marcelo Emílio⁶,de Figueiredo José Ricardo¹^ORCID

Affiliation:

1. Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA) State University of Ceará Fortaleza Brazil

2. Federal Rural University of the Amazon Paragominas Brazil

3. Federal University of Jataí Jataí Brazil

4. Conception Biosciences Inc Berkeley California USA

5. Federal University of Tocantins Palmas Brazil

6. Laboratory Reproductive Biology Institute of Biomedicine, Federal University of Uberlandia Uberlandia Brazil

Abstract

AbstractThis work investigated the effect of zinc oxide nanoparticles functionalized with curcumin (ZnO(np) + CUR) supplementation during the in vitro embryo culture (IVC) on the bovine in vitro embryo production, and the cellular antioxidant response. The cumulus–oocyte complexes (COCs) were matured, fertilized and then the presumptive zygotes were cultured in the medium in the absence (0 μM—control) or presence of different concentrations of ZnO(np) + CUR (3, 6 or 12 μM). After IVC, the embryos were destined either to assay intracellular ROS levels and mitochondrial membrane potential. The results demonstrated that only the addition of 12 μM ZnO(np) + CUR during IVC decreased intracellular ROS production and the rate of blastocyst production when compared to the control (p < .05). In conclusion, ZnO(np) + CUR addition during the IVC impaired in concentration‐dependent‐manner bovine in vitro embryo production.

Funder

Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico

Publisher

Wiley

Subject

Endocrinology,Animal Science and Zoology,Biotechnology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1111/rda.14416

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