Boar sperm motility is modulated by CCK at a low concentration of bicarbonate under capacitation conditions

Author:

Moros‐Nicolás C.12ORCID,López‐Úbeda R.12,Luongo C.23,Sòria‐Monzó P.12,Abril‐Sánchez S.3,Grudzinska P.3,Avilés M.12,Izquierdo‐Rico M. J.12,García‐Vázquez F. A.23ORCID

Affiliation:

1. Departamento de Biología Celular e Histología, Facultad de Medicina, Campus de Ciencias de la Salud, Campus Mare Nostrum (CMN) Universidad de Murcia Murcia Spain

2. Instituto Murciano de Investigación Biosanitaria Pascual Parrilla (IMIB) Campus de Ciencias de la Salud Murcia Spain

3. Departamento de Fisiología, Facultad de Veterinaria, Campus de Espinardo, Campus Mare Nostrum (CMN) Universidad de Murcia Murcia Spain

Abstract

AbstractIn a previous study, our group detected the cholecystokinin (CCK) protein in the porcine oviduct. This fact, together with the involvement of CCK in the regulation of sperm protein tyrosine phosphorylation by the modulation of HCO3 uptake (in mice and humans) suggests a role for CCK during sperm capacitation. Therefore, on the one hand, the expression of CCK receptors (CCK1R and CCK2R) on boar testes has been investigated and probed; on the other hand, boar spermatozoa (from seminal doses of 1‐day and 5‐day storage) were exposed to different concentrations of CCK (0‐control, 25 or 50 μM) in a medium supporting capacitation supplemented with 0, 5 or 25 mmol/L of HCO3 for 1 h at 38.5°C. Sperm motion (total and progressive motility), kinetic parameters, viability, acrosome status, and mitochondrial activity were determined. No differences between groups (0, 25 or 50 μM of CCK) were observed when HCO3 was absent in the media (p > .05). However, the results showed that when the media was supplemented with 5 mmol/L HCO3 in 1‐day seminal dose storage, the linearity index (LIN, %), straightness index (STR, %) and oscillation index (WOB, %) (sperm kinetics parameters) increased in the presence of CCK regardless the concentration (p < .05). Nevertheless, CCK in sperm from 5‐day storage only increased the WOB parameter in comparison to the control (p < .05). Furthermore, the average amplitude of the lateral displacement of the sperm head (ALH, μm) and curvilinear velocity (VCL, μm/s) decreased when CCK was present, depending on its concentration and sperm aging (1‐day vs. 5‐days) (p < .05). In the case of the media supporting capacitation supplemented with 25 mmol/L HCO3, any differences were observed except for sperm viability in the 5‐day seminal doses, which increased in the 50 μM‐CCK group compared to the control (p < .05). In conclusion, these data suggest an implication of CCK protein during sperm capacitation under low bicarbonate concentration increasing the sperm linear trajectory.

Funder

European Regional Development Fund

Ministerio de Ciencia e Innovación

Publisher

Wiley

Subject

Endocrinology,Animal Science and Zoology,Biotechnology

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