GTSE1 promotes the growth of NSCLC by regulating microtubule‐associated proteins through the ERK/MAPK pathway

Author:

Wang Chuanlin12ORCID,Wen Meiyan12,Xu Jiali12,Gao Pengning12,Liu Shanling12,Liu Jiayu3,Chen Ying24ORCID,Zhou Lan12ORCID

Affiliation:

1. Department of Clinical Nutrition Yunnan Cancer Hospital/The Third Affiliated Hospital of Kunming Medical University Kunming China

2. Department of Clinical Nutrition Yunnan Cancer Center Kunming China

3. Key Laboratory of Environmental Toxicology of Anhui Higher Education Institutes, Department of Toxicology, School of Public Health Anhui Medical University Hefei China

4. Department of Thoracic Surgery Yunnan Cancer Hospital/The Third Affiliated Hospital of Kunming Medical University Kunming China

Abstract

AbstractThe role of G2 and S phase‐expressed‐1 (GTSE1), a microtubule‐localized protein, in non‐small‐cell lung cancer (NSCLC) remains unknown. We explored its role in NSCLC growth. GTSE1 was detected in NSCLC tissues and cell lines using quantitative real‐time polymerase chain reaction. The clinical significance of GTSE1 levels was evaluated. Biological and apoptotic effects of GTSE1 were evaluated using transwell, cell‐scratch, and MTT assays, and flow cytometry and western blotting, respectively. Its association with cellular microtubules was shown by western blotting and immunofluorescence. GTSE1 expression was upregulated in NSCLC tissues and cell lines. GTSE1 levels correlated with lymph node metastasis. Higher GTSE1 mRNA expression correlated with shorter progression‐free survival. GTSE1‐knockdown decreased proliferation, colony formation, invasion, and migration of NSCLC cells, and inhibited tau and stathmin‐1 microtubule‐associated protein expression, via the extracellular‐regulated protein kinase/mitogen‐activated protein kinase (ERK/MAPK) signaling pathway, and microtubule disruption. GTSE1 may promote NSCLC growth by regulating tau and stathmin‐1 through the ERK/MAPK signaling pathway.

Funder

Yunnan Provincial Science and Technology Department

Publisher

Wiley

Subject

Pulmonary and Respiratory Medicine,Oncology,General Medicine

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