Affiliation:
1. Department of Respiratory and Critical Care Medicine West China Hospital of Sichuan University Chengdu China
2. Division of Pulmonary Diseases, State Key Laboratory of Biotherapy of China West China Hospital, West China School of Medicine, Sichuan University Chengdu China
3. School of Medical and Life Sciences Chengdu University of Traditional Chinese Medicine Chengdu China
4. Key Laboratory of Acupuncture for Senile Disease (Chengdu University of TCM), Ministry of Education Chengdu China
5. Department of Respiratory and Critical Care Medicine Chengdu Fifth People's Hospital Chengdu China
Abstract
AbstractWe used 10 × genomics single‐cell transcriptome sequencing technology to reveal the tumor immune microenvironment characteristics of small cell lung cancer (SCLC) in a patient with malignant pleural effusion (MPE). A total of 8008 high‐quality cells were finally obtained for subsequent bioinformatic analysis, which were divided into 10 cell clusters further identified as B cells, T cells, myeloid cells, NK cells, and cancer cells. Such SCLC related genes as NOTCH1, MYC, TSC22D1, SOX4, BLNK, YBX3, VIM, CD8A, CD8B, and KLF6 were expressed in different degrees during differentiation of T and B cells. Different ligands and receptors between T, B and tumor cells almost interact through MHC II, IL‐16, galectin, and APP signaling pathway.
Funder
National Natural Science Foundation of China
Subject
Pulmonary and Respiratory Medicine,Oncology,General Medicine