Characterization of the 263K‐derived microsomal fraction: a source of prions for nanofiltration validation studies

Author:

Simoneau Steve1ORCID,Igel Angélique2,Ciric Danica2,Moudjou Mohammed2,Tcherniuk Sergey1,Béringue Vincent2,Rezaei Human2,Flan Benoit1

Affiliation:

1. Department of Biological Safety Surveillance Laboratoire du Fractionnement et des Biotechnologies (LFB) Les Ulis France

2. VIM, INRAE, Paris‐Saclay University Jouy‐en‐Josas France

Abstract

AbstractBackgroundThe manufacturing processes of plasma products include steps that can remove prions. The efficacy of these steps is measured in validation studies using animal brain‐derived prion materials called spikes. Because the nature of the prion agent in blood is not known, the relevance of these spikes, particularly with steps that are based on retention mechanisms such as nanofiltration, is important to investigate.Study Design and MethodsThe aggregation and sizes of PrPres assemblies of microsomal fractions (MFs) extracted from 263K‐infected hamster brains were analyzed using velocity gradients. The separated gradient fractions were either inoculated to Tg7 mice expressing hamster‐PrPc to measure infectivity or used in Protein Misfolding Cyclic Amplification for measuring seeding activity. The collected data allowed for reanalyzing results from previous nanofiltration validation studies.ResultsA significant portion of MFs was found to be composed of small PrPres assemblies, estimated to have a size ≤24 mers (~22–528 kDa), and to contain a minimum of 20% of total prion infectivity. With this data we could calculate reductions of 4.10 log (15 N), 2.53 log (35 N), and 1.77 log (35 N) from validation studies specifically for these small PrPres objects.ConclusionOur gradient data provided evidence that nanofilters can remove the majority of the smallest PrPres entities within microsomes spikes, estimated to be in a size below 24 mers, giving insight about the fact that, in our conditions, size exclusion may not be the only mechanism for retention nanofiltration.

Publisher

Wiley

Reference40 articles.

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