The SCOOP‐MIK2 immune pathway modulates Arabidopsis root growth and development by regulating PINFORMED abundance and auxin transport

Author:

Wang Xian1,Chen Meng1,Li Jie1,Kong Mengjuan1,Tan Shutang1ORCID

Affiliation:

1. MOE Key Laboratory for Cellular Dynamics, Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Hefei National Laboratory for Physical Sciences at the Microscale, Division of Life Sciences and Medicine University of Science and Technology of China Hefei 230027 China

Abstract

SUMMARYPlants synthesize hundreds of small secretory peptides, which are perceived by the receptor‐like kinase (RLK) family at the cell surface. Various signaling peptide‐RLK pairs ensure plant adaptation to distinct environmental conditions. Here, we report that SERINE RICH ENDOGENOUS PEPTIDE (SCOOP) immune peptides modulate root growth and development by regulating PIN‐FORMED (PIN)‐regulated polar auxin transport in Arabidopsis. The SCOOP4 and SCOOP12 treatments impaired root gravitropic growth, auxin redistribution in response to gravistimulation, and PIN abundance in the PM. Furthermore, genetic and cell biological analyses revealed that these physiological and cellular effects of SCOOP4 and SCOOP12 peptides are mediated by the receptor MALE DISCOVERER1‐INTERACTING RECEPTOR LIKE KINASE2 (MIK2) and the downstream mitogen‐activated kinase MPK6. Biochemical evidence indicates that MPK6 directly phosphorylates the cytosolic loop of PIN proteins. Our work established a link between the immune signaling peptide SCOOPs and root growth pathways, providing insights into the molecular mechanisms underlying plant root adaptive growth in the defense response.

Funder

National Natural Science Foundation of China

Publisher

Wiley

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