Improving transient protein expression in agroinfiltrated Nicotiana benthamiana

Author:

Beritza Konstantina1ORCID,Watts Emma C.1ORCID,van der Hoorn Renier A. L.1ORCID

Affiliation:

1. The Plant Chemetics Laboratory, Department of Biology University of Oxford OX1 3RB Oxford UK

Abstract

SummaryAgroinfiltration of Nicotiana benthamiana is routinely used in plant science and molecular pharming to transiently express proteins of interest. Here, we discuss four phenomena that should be avoided to improve transient expression. Immune responses can be avoided by depleting immune receptors and employing pathogen‐derived effectors; transcript degradation by using silencing inhibitors or RNA interference machinery mutants; endoplasmic reticulum stress by co‐expressing chaperones; and protein degradation can be avoided with subcellular targeting, protease mutants and co‐expressing protease inhibitors. We summarise the reported increased yields for various recombinant proteins achieved with these approaches and highlight remaining challenges to further improve the efficiency of this versatile protein expression platform.

Funder

Biotechnology and Biological Sciences Research Council

European Research Council

Publisher

Wiley

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