Affiliation:
1. College of Medical Imaging Xuzhou Medical University Xuzhou Jiangsu China
2. Blood Diseases Institute Xuzhou Medical University Xuzhou Jiangsu China
3. The Second Affiliated Hospital of Xuzhou Medical University Xuzhou Jiangsu China
Abstract
AbstractMultiple myeloma (MM) is a prevalent plasma cell malignancy in the blood system that remains incurable. Given the abnormally high expression of c‐Maf in most MM patients, targeting c‐Maf presents an attractive therapeutic approach for treating MM malignancies. In this study, we employed a combined strategy involving molecular docking‐based virtual screening, molecular dynamics (MD) simulation, and molecular mechanics/generalized Born surface area (MM/GBSA) free energy calculation on existing FDA‐approved drugs. Six compounds were selected for further experimental assay: vemurafenib, sorafenib, sildenafil, fluvastatin, erlotinib, and glimepiride. Among these compounds, sorafenib and glimepiride exhibited significant inhibition of myeloma cell proliferation in the RPMI‐8226 cell line. Moreover, both compounds simultaneously downregulated c‐Maf protein expression to induce G1 phase arrest and apoptosis in myeloma cells. Collectively, sorafenib and glimepiride may be considered promising candidates for developing more potent c‐Maf inhibitors in the future.
Subject
Molecular Medicine,Biochemistry,Drug Discovery,Pharmacology,Organic Chemistry