Molecular characterization of mungbean yellow mosaic India virus infecting Vigna radiata in Oman

Abstract

AbstractThe full‐genome sequences of a bipartite begomovirus were identified in a mungbean (Vigna radiata) crop displaying symptoms such as yellow mosaics and stunting in the Al‐Batinah North region (23.6867° N 57.9058° E), Oman. Initial detection and confirmation of the virus genome were carried out through polymerase chain reaction, followed by obtaining complete genomes using rolling circle amplification. Bioinformatics analysis on both genomic components, DNA‐A and DNA‐B, revealed over 99% nucleotide sequence identity to the mungbean yellow mosaic India virus (MYMIV), previously known to infect tomato plants. Pairwise sequence analysis using the STD tool and subsequent phylogenetic analysis unveiled that the DNA‐A of the MYMIV isolate formed a cluster with closely related DNA‐A sequences of MYMIV from GenBank, while the DNA‐B clustered with the corresponding DNA‐B of the MYMIV isolate. Additionally, no evidence of recombination events was observed in the recombination analysis. Similarly, the nucleotide substitution rates between the DNA‐A and DNA‐B segments of MYMIV did not show significant differences. This finding represents the first documented instance of bipartite MYMIV infecting V. radiata in Oman.