Novel synthetic inducible promoters controlling gene expression during water‐deficit stress with green tissue specificity in transgenic poplar

Author:

Yang Yongil1,Chaffin Timothy A.1,Shao Yuanhua12,Balasubramanian Vimal K.3,Markillie Meng3,Mitchell Hugh3,Rubio‐Wilhelmi Maria M.4,Ahkami Amir H.3,Blumwald Eduardo4ORCID,Neal Stewart C.12ORCID

Affiliation:

1. Center for Agricultural Synthetic Biology University of Tennessee Institute of Agriculture Knoxville Tennessee USA

2. Department of Plant Sciences University of Tennessee Knoxville Tennessee USA

3. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory Richland WA USA

4. Department of Plant Sciences University of California Davis California USA

Abstract

SummarySynthetic promoters may be designed using short cis‐regulatory elements (CREs) and core promoter sequences for specific purposes. We identified novel conserved DNA motifs from the promoter sequences of leaf palisade and vascular cell type‐specific expressed genes in water‐deficit stressed poplar (Populus tremula × Populus alba), collected through low‐input RNA‐seq analysis using laser capture microdissection. Hexamerized sequences of four conserved 20‐base motifs were inserted into each synthetic promoter construct. Two of these synthetic promoters (Syn2 and Syn3) induced GFP in transformed poplar mesophyll protoplasts incubated in 0.5 M mannitol solution. To identify effect of length and sequence from a valuable 20 base motif, 5′ and 3′ regions from a basic sequence (GTTAACTTCAGGGCCTGTGG) of Syn3 were hexamerized to generate two shorter synthetic promoters, Syn3‐10b‐1 (5′: GTTAACTTCA) and Syn3‐10b‐2 (3′: GGGCCTGTGG). These promoters' activities were compared with Syn3 in plants. Syn3 and Syn3‐10b‐1 were specifically induced in transient agroinfiltrated Nicotiana benthamiana leaves in water cessation for 3 days. In stable transgenic poplar, Syn3 presented as a constitutive promoter but had the highest activity in leaves. Syn3‐10b‐1 had stronger induction in green tissues under water‐deficit stress conditions than mock control. Therefore, a synthetic promoter containing the 5′ sequence of Syn3 endowed both tissue‐specificity and water‐deficit inducibility in transgenic poplar, whereas the 3′ sequence did not. Consequently, we have added two new synthetic promoters to the poplar engineering toolkit: Syn3‐10b‐1, a green tissue‐specific and water‐deficit stress‐induced promoter, and Syn3, a green tissue‐preferential constitutive promoter.

Funder

Basic Energy Sciences

Publisher

Wiley

Subject

Plant Science,Agronomy and Crop Science,Biotechnology

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