Syntaxin 3 SPI‐2 dependent crosstalk facilitates the division of Salmonella containing vacuole

Author:

Chatterjee Ritika1ORCID,Nair Abhilash Vijay1,Singh Anmol1,Mehta Nishi1,Setty Subba Rao Gangi1ORCID,Chakravortty Dipshikha12ORCID

Affiliation:

1. Department of Microbiology and Cell Biology, Division of Biological Sciences Indian Institute of Science Bangalore India

2. School of Biology Indian Institute of Science Education and Research Thiruvananthapuram Kerala India

Abstract

AbstractIntracellular membrane fusion is mediated by membrane‐bridging complexes of soluble N‐ethylmaleimide‐sensitive factor attachment protein receptors (SNAREs). SNARE proteins are one of the key players in vesicular transport. Several reports shed light on intracellular bacteria modulating host SNARE machinery to establish infection successfully. The critical SNAREs in macrophages responsible for phagosome maturation are Syntaxin 3 (STX3) and Syntaxin 4 (STX4). Reports also suggest that Salmonella actively modulates its vacuole membrane composition to escape lysosomal fusion. Salmonella containing vacuole (SCV) harbours recycling endosomal SNARE Syntaxin 12 (STX12). However, the role of host SNAREs in SCV biogenesis and pathogenesis remains unclear. Upon knockdown of STX3, we observed a reduction in bacterial proliferation, which is concomitantly restored upon the overexpression of STX3. Live‐cell imaging of Salmonella‐infected cells showed that STX3 localises to the SCV membranes and thus might help in the fusion of SCV with intracellular vesicles to acquire membrane for its division. We also found the interaction STX3‐SCV was abrogated when we infected with SPI‐2 encoded Type 3 secretion system (T3SS) apparatus mutant (STM ∆ssaV) but not with SPI‐1 encoded T3SS apparatus mutant (STM ∆invC). These observations were also consistent in the mice model of Salmonella infection. Together, these results shed light on the effector molecules secreted through T3SS encoded by SPI‐2, possibly involved in interaction with host SNARE STX3, which is essential to maintain the division of Salmonella in SCV and help to maintain a single bacterium per vacuole.

Funder

Department of Atomic Energy, Government of India

Department of Biotechnology, Ministry of Science and Technology, India

Department of Science and Technology, Ministry of Science and Technology, India

Indian Council of Medical Research

Department of Science and Technology, Government of West Bengal

Science and Engineering Research Board

The Wellcome Trust DBT India Alliance

Publisher

Wiley

Subject

Cell Biology,Genetics,Molecular Biology,Biochemistry,Structural Biology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3