Bacillus subtilis promotes plant phosphorus (P) acquisition through P solubilization and stimulation of root and root hair growth

Author:

Jensen Camilla Niketa Gadomska12ORCID,Pang Janet Ka Yan3,Gottardi Michele2,Kračun Stjepan Krešimir4ORCID,Svendsen Birgit Albrecht4,Nielsen Kristian Fog5ORCID,Kovács Ákos T.36ORCID,Moelbak Lars2,Fimognari Lorenzo2ORCID,Husted Søren1ORCID,Schulz Alexander1ORCID

Affiliation:

1. Department of Plant and Environmental Sciences University of Copenhagen Frederiksberg Denmark

2. Plant Health Innovation Novonesis A/S Taastrup Denmark

3. Bacterial Interactions and Evolution Group, DTU Bioengineering Technical University of Denmark Kgs. Lyngby Denmark

4. R&D Microbial Screening R&D Microbe & Culture Research Novonesis A/S Hørsholm Denmark

5. Biochemical Assays Novonesis A/S Hørsholm Denmark

6. Institute of Biology Leiden University Leiden the Netherlands

Abstract

AbstractBacteria can be applied as biofertilizers to improve crop growth in phosphorus (P)‐limited conditions. However, their mode of action in a soil environment is still elusive. We used the strain ALC_02 as a case study to elucidate how Bacillus subtilis affects dwarf tomato cultivated in soil‐filled rhizoboxes over time. ALC_02 improved plant P acquisition by increasing the size and P content of P‐limited plants. We assessed three possible mechanisms, namely root growth stimulation, root hair elongation, and solubilization of soil P. ALC_02 produced auxin, and inoculation with ALC_02 promoted root growth. ALC_02 promoted root hair elongation as the earliest observed response and colonized root hairs specifically. Root and root hair growth stimulation was associated with a subsequent increase in plant P content, indicating that a better soil exploration by the root system improved plant P acquisition. Furthermore, ALC_02 affected the plant‐available P content in sterilized soil differently over time and released P from native P pools in the soil. Collectively, ALC_02 exhibited all three mechanisms in a soil environment. To our knowledge, bacterial P biofertilizers have not been reported to colonize and elongate root hairs in the soil so far, and we propose that these traits contribute to the overall effect of ALC_02. The knowledge gained in this research can be applied in the future quest for bacterial P biofertilizers, where we recommend assessing all three parameters, not only root growth and P solubilization, but also root hair elongation. This will ultimately support the development of sustainable agricultural practices.

Funder

Novo Nordisk Fonden

Innovation Fund

Publisher

Wiley

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