Enhancing classical swine fever virus identification: the advantages of Field‐LAMP testing

Abstract

Classical swine fever virus (CSFV) identification has witnessed significant advancements with the development of rapid reverse‐transcription loop‐mediated isothermal amplification (RT‐LAMP) assays. However, conventional RT‐LAMP assays for CSFV diagnosis are hindered by a laborious RNA extraction step. Moreover, the need for thermal incubators and expensive micropipettes has limited their application in field settings. Addressing these challenges, our study presents a groundbreaking solution—an electro‐free and point‐of‐care (POC) tool known as the field‐LAMP assay—for the rapid clinical detection of CSFV. By eliminating the RNA extraction requirement, advancing the colorimetric read‐out and lyophilized reaction reagents, our field‐LAMP assay streamlines the diagnostic process, saving valuable time and effort. This novel approach also overcomes the dependency on electric‐dependent thermal incubators and expensive micropipettes, making it practical and accessible for use in the field. The successful development of the field‐LAMP assay marks a significant milestone in CSFV detection. This electro‐free and POC tool offers several advantages, including its ability to deliver rapid results without compromising accuracy, facilitating prompt response and containment measures.