Comparison of ionised calcium measured using a portable analyser to a reference method in healthy dogs

Author:

Harper J. A.1,Gal A.2,Burchell R. K.1ORCID,Summers J. D.3,Starling J.4,Gerber K.4,Gummow B.4

Affiliation:

1. North Coast Specialist and Referral Centre 5/30 Chancellor Village, Sippy Downs, Sunshine Coast Australia

2. Department of Veterinary Clinical Medicine College of Veterinary Medicine, Carle Illinois College of Medicine, University of Illinois Urbana Illinois USA

3. Gracemere Veterinary Surgery 22 Laurie Street, Gracemere 4702 Australia

4. College of Public Health, Medical and Veterinary Sciences James Cook University Townsville Australia

Abstract

ObjectivesTo compare the ionised calcium measured on a portable analyser (iSTAT, Abbott) to a reference method.Materials and MethodsBlood samples from 39 apparently healthy dogs were analysed in duplicate using a portable analyser and a reference method (Radiometer ABL800 FLEX). Bland–Altman plots and Passing–Bablok regression were used to assess constant and proportional bias between the two instruments. A within‐assay percentage coefficient of variation and total error (TE) was calculated for both analysers. The reference interval was calculated for the portable analyser using the robust method with confidence interval bootstrapping.ResultsThe Bland–Altman plot showed a −0.036 mmol/L difference between the two instruments (95% confidence limit −0.08 to 0.01 mmol/L; limits of agreement −0.07 to 0.006 mmol/L). Neither the Bland–Altman plot nor the Passing–Bablock regression (slope −0.03; 95% confidence interval −0.08 to 0.19 and intercept 1; 95% confidence interval 0.83 to 1.2) showed significant proportional bias. The coefficient of variation for the portable analyser was 1.08%, compared to 0.78% for the reference method with a total error of 3.5% for the portable analyser. The estimated population‐based reference interval for ionised calcium using the portable analyser is 1.23 to 1.42 mmol/L.Clinical SignificanceFor the healthy dogs in this study, compared to the reference method, the portable analyser showed no significant bias for measurement of ionised calcium. Further studies including hyper and hypocalcaemic dogs are required to determine clinical impact of the use of this analyser.

Publisher

Wiley

Subject

Small Animals

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