Combination of transcriptomic, proteomic, and degradomic profiling reveals common and distinct patterns of pathogen‐induced cell death in maize

Author:

Barghahn Sina1,Saridis Georgios1,Mantz Melissa23,Meyer Ute1,Mellüh Jaqueline C.1,Misas Villamil Johana C.14,Huesgen Pitter F.235,Doehlemann Gunther14ORCID

Affiliation:

1. Institute for Plant Sciences University of Cologne Cologne Germany

2. Central Institute for Engineering Electronics and Analytics, ZEA‐3 Forschungszentrum Jülich Jülich Germany

3. Cologne Excellence Cluster on Cellular Stress Responses in Aging‐Associated Diseases (CECAD) Medical Faculty and University Hospital, University of Cologne Cologne Germany

4. Cluster of Excellence on Plant Sciences (CEPLAS) University of Cologne Cologne Germany

5. Institute of Biochemistry University of Cologne Cologne Germany

Abstract

SUMMARYRegulated cell death (RCD) is crucial for plant development, as well as in decision‐making in plant‐microbe interactions. Previous studies revealed components of the molecular network controlling RCD, including different proteases. However, the identity, the proteolytic network as well as molecular components involved in the initiation and execution of distinct plant RCD processes, still remain largely elusive. In this study, we analyzed the transcriptome, proteome, and N‐terminome of Zea mays leaves treated with the Xanthomonas effector avrRxo1, the mycotoxin Fumonisin B1 (FB1), or the phytohormone salicylic acid (SA) to dissect plant cellular processes related to cell death and plant immunity. We found highly distinct and time‐dependent biological processes being activated on transcriptional and proteome levels in response to avrRxo1, FB1, and SA. Correlation analysis of the transcriptome and proteome identified general, as well as trigger‐specific markers for cell death in Zea mays. We found that proteases, particularly papain‐like cysteine proteases, are specifically regulated during RCD. Collectively, this study characterizes distinct RCD responses in Z. mays and provides a framework for the mechanistic exploration of components involved in the initiation and execution of cell death.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Wiley

Subject

Cell Biology,Plant Science,Genetics

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